Insight into cellular proteome of Lolium multiflorum/Festuca arundinacea introgression forms to decipher crucial mechanisms of cold acclimation in forage grasses. (July 2018)

• Record Type: Journal Article
• Authors: Augustyniak, Adam; Perlikowski, Dawid; Rapacz, Marcin; Kościelniak, Janusz; Kosmala, Arkadiusz
• Is Part Of: Plant science. Volume 272(2018)
• Page Start: 22
• Page End: 31
• Publication Date: 2018-07
• Subjects: ABS/CS energy flux for absorbed energy/area of the photosynthetic sample -- ABS/RC energy flux for absorbed energy/reaction center -- CA cold acclimation -- 2-DE two-dimensional electrophoresis -- DI0/CS energy flux for dissipated energy/area of the photosynthetic sample -- DI0/RC energy flux for dissipated energy/reaction center -- E transpiration -- EL electrolyte leakage -- ETo/CS energy flux for electron transport/area of the photosynthetic sample -- ET0/RC energy flux for electron transport/reaction center -- Fv/Fm maximum quantum yield of primary photochemistry -- HFT high frost tolerant -- IEF isoelectrofocusing -- LFT low frost tolerant -- MS mass spectrometry -- PItotal performance index (potential) for energy conservation from exciton to the reduction of PSI end acceptors -- Pn (A) net photosynthesis (assimilation of CO2) -- PPFD photosynthetic photon flux density -- PS photosystem -- RC/CSm densities of active PSII reaction centers at tmax (time to reach maximum fluorescence) -- RC/CS0 densities of active PSII reaction centers at t = 0 -- RE0/RC electron flux reducing end electron acceptors at the PSI acceptor side/reaction center -- TEL50 temperature causing a 50% electrolyte leakage -- TRo/CS energy flux for trapped energy/area of the photosynthetic sample -- TRo/RC energy flux for trapped energy/reaction center -- %Vol mean of normalized protein spot volumes -- WUE water use efficiency -- φEo quantum yield of electron transport -- ψo overall quantum yield between light absorption and the electron transport beyond quinone A -- φRo quantum yield for reduction of end electron acceptors at the PSI acceptor side; Aldolase -- Calvin cycle -- Cellular membrane integrity -- Cold acclimation -- Frost tolerance -- Photosynthesis; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.04.002
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Cold acclimation of photosynthetic apparatus is crucial to improve frost tolerance in Lolium-Festuca hybrids. The capacity to acclimate photosynthesis to low temperature could be controlled by the efficiency of carbon assimilation and metabolism. Accumulation and activity of different forms of chloroplast aldolase trigger a course of the Calvin cycle. Abstract: Frost tolerance is the main component of winter-hardiness. To express this trait, plants sense low temperature, and respond by activating the process of cold acclimation. The molecular mechanisms of this acclimation have not been fully understood in the agronomically important group of forage grasses, including Lolium-Festuca species. Herein, the introgression forms of L. multiflorum / F. arundinacea distinct with respect to their frost tolerance, were used as models for the comprehensive, proteomic and physiological, research to recognize the crucial components of cold acclimation in forage grasses. The obtained results stressed the importance of photosynthetic performance under acclimation to low temperature. The stable level of photochemical processes after three weeks of cold acclimation in the introgression form with a higher level of frost tolerance, combined simultaneously with only slightly (but not significantly) decreased level of CO2 assimilation after that period, despite significantly lower stomatal conductance, indicated the capacity for that form to acclimate its photosynthesis to low temperature. This phenomenon was driven by the Calvin cycle efficiency, associated with revealed here accumulation profiles and activities of chloroplastic aldolase. The capacity to acclimate the photosynthetic machinery to cold could be one of the most crucial components of forage grass metabolism to improve frost tolerance.