Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses. (July 2018)

• Record Type: Journal Article
• Authors: Navarro-León, Eloy; Ruiz, Juan Manuel; Graham, Neil; Blasco, Begoña
• Is Part Of: Plant science. Volume 272(2018)
• Page Start: 164
• Page End: 172
• Publication Date: 2018-07
• Subjects: APX ascorbate peroxidase -- CaUpE Ca uptake efficiency -- CaUtE Ca utilisation efficiency -- CAX cation/H+ exchanger -- Chl chlorophyll -- DC distribution coefficient -- GMOs genetically modified organisms -- LOX lipoxygenase -- MDA malondialdehyde -- ROS reactive oxygen species -- TILLING targeting induced local lesions in genomes; Brassica rapa -- Calcium -- CAX1 -- Chl fluorescence -- Ionome -- Oxidative stress -- Physiological profile -- TILLING; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.04.019
• Languages: English
• ISSNs: 0168-9452

Abstract

Graphical abstract: Highlights: BraA.cax1a mutants appear to be unhelpful to better Ca-deficiency tolerance. BraA.cax1a-4 and BraA.cax1a-7 showed stress symptoms in comparison to R-o-18. Mutants are useful for biofortification as accumulated more Ca, Mg and Fe in leaves. BraA.cax1a-12 mutation allows a better growth under high Ca conditions. BraA.cax1a-12 could be useful in biofortification and phytoremediation programs. Abstract: Calcium (Ca) is an essential macronutrient for plants and its homeostasis is basic for many processes in plants. Therefore, both Ca deficiency and toxicity constitute potential issues for crops. CAX1 transporter is a potential target to obtain plants with better Ca homeostasis and higher Ca concentration in edible parts. Three Brassica rapa mutants for CAX1 were obtained through TILLING. The objective of this work is to evaluate the growth, physiological state and nutrients concentration of these mutants grown with different Ca doses. The mutants and the parental line were grown under low, control and high Ca doses and parameters related to their oxidative stress, photosynthetic performance and nutrients concentration were determined. BraA.cax1a-4 and BraA.cax1a-7 mutants presented lower total Chl, an altered photosynthesis performance and higher ROS levels. BraA.cax1a-12 mutant grew better under high Ca conditions. All mutants accumulated more Ca and Mg in leaves under control and high Ca doses and accumulated more Fe regardless the Ca dose. The results obtained point to BraA.cax1a-12 as a potential candidate for biofortification with Fe, Ca and Mg since it accumulate higher concentrations of these elements, do not present an altered growth and is able to tolerate higher Ca doses.