An Amperometric Glucose Biosensor Based on Poly (Pyrrole‐2‐Carboxylic Acid)/Glucose Oxidase Biocomposite. Issue 8 (23rd March 2018)

• Record Type: Journal Article
• Authors: Kausaite‐Minkstimiene, Asta; Glumbokaite, Laura; Ramanaviciene, Almira; Dauksaite, Elena; Ramanavicius, Arunas
• Is Part Of: Electroanalysis. Volume 30:Issue 8(2018)
• Page Start: 1642
• Page End: 1652
• Publication Date: 2018-03-23
• Subjects: Amperometric glucose biosensors -- Glucose oxidase -- Poly (pyrrole-2-carboxylic acid) -- Conjugated polymers -- Electrochemical biosensors; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201800044
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A newly developed amperometric glucose biosensor based on graphite rod (GR) working electrode modified with biocomposite consisting of poly (pyrrole‐2‐carboxylic acid) (PCPy) particles and enzyme glucose oxidase (GOx) was investigated. The PCPy particles were synthesized by chemical oxidative polymerization technique using H2 O2 as initiator of polymerization reaction and modified covalently with the GOx (PCPy‐GOx) after activation of carboxyl groups located on the particles surface with a mixture of N‐(3‐dimethylaminopropyl)‐N′‐ethylcarbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS). Then the PCPy‐GOx biocomposite was dispersed in a buffer solution containing a certain amount of bovine serum albumin (BSA). The resulting biocomposite suspension was adsorbed the on GR electrode surface with subsequent solvent airing and chemical cross‐linking of the proteins with glutaraldehyde vapour (GR/PCPy‐GOx). It was determined that the current response of the GR/PCPy‐GOx electrodes to glucose measured at +300 mV vs Cl reference electrode was influenced by the duration of the PCPy particles synthesis, pH of the GOx solution used for the PCPy particles modification and the amount of immobilized PCPy‐GOx biocomposite. An optimal pH of buffer solution for operation of the biosensor was found to be 8.0. Detection limit was determined as 0.039 mmol L −1 according signal to noise ratio (S/N: 3). The proposed glucose biosensor was tested in human serum samples. Abstract :