Inhibitory Effect of Phthalic Acid on Tyrosinase: The Mixed-Type Inhibition and Docking Simulations. (23rd May 2011)

• Record Type: Journal Article
• Authors: Yin, Shang-Jun; Si, Yue-Xiu; Qian, Guo-Ying
• Other Names: Park Yong-Doo Academic Editor.
• Is Part Of: Enzyme research. Volume 2011(2011)
• Publication Date: 2011-05-23
• Subjects: Enzymes -- Periodicals; 572.7
• DOI: 10.4061/2011/294724
• Languages: English
• ISSNs: 2090-0406

Abstract

Abstract : Tyrosinase inhibition studies are needed due to the medicinal applications such as hyperpigmentation. For probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics was important. We predicted the 3D structure of tyrosinase, used a docking algorithm to simulate binding between tyrosinase and phthalic acid (PA), and studied the reversible inhibition of tyrosinase by PA. PA inhibited tyrosinase in a mixed-type manner with aK i = 65.84 ± 1.10 mM. Measurements of intrinsic and ANS-binding fluorescences showed that PA induced changes in the active site structure via indirect binding. Simulation was successful (binding energies forDock 6.3 = - 27.22 andAutoDock 4.2 = - 0.97 kcal/mol), suggesting that PA interacts with LEU73 residue that is predicted commonly by both programs. The present study suggested that the strategy of predicting tyrosinase inhibition based on hydroxyl groups and orientation may prove useful for screening of potential tyrosinase inhibitors.