Development of "Quick-DB forensic": A total workflow from QuEChERS-dSPE method to GC–MS/MS quantification of forensically relevant drugs and pesticides in whole blood. (July 2019)

• Record Type: Journal Article
• Authors: Kusano, Maiko; Sakamoto, Yuki; Natori, Yujin; Miyagawa, Haruhiko; Tsuchihashi, Hitoshi; Ishii, Akira; Zaitsu, Kei
• Is Part Of: Forensic science international. Volume 300(2019)
• Page Start: 125
• Page End: 135
• Publication Date: 2019-07
• Subjects: GC-MS/MS -- QuEChERS -- Quick-DB forensic; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2019.03.048
• Languages: English
• ISSNs: 0379-0738

Abstract

Highlights: Simultaneous determination and quantification of 63 forensically relevant drugs and pesticide in whole blood. Total workflow from QuEChERS-dSPE extraction to GC–MS/MS analysis easily operable for naïve to experienced analysts. Development of "Quick-DB Forensic" software for rapid identification and quantitation by GC–MS/MS. Abstract: Trends in forensic toxicology show the advancement of rapid and sensitive analytical methods for qualitative and quantitative analysis of drugs of abuse. However, forensic toxicologists are continuously faced with the challenges of identifying and quantifying drug blood concentration while simultaneously struggling with manpower shortage. In view of developing a simple and productive toxicological analysis method encompassing total workflow from sample preparation to quantitative analysis, here we describe a simple, robust, and sensitive method for the simultaneous determination and quantification of 63 forensically relevant drugs and pesticides in human whole blood. The method is based on sample preparation by a modified QuEChERS extraction and dispersive solid-phase extraction (dSPE) clean-up followed by gas chromatography–tandem mass spectrometry (GC–MS/MS) analysis. Limits of detection of the target analytes in whole blood ranged in the few ng/mL-order levels. Intra- and inter-day validation result ranges were 0–24% for accuracy (% error) and 0.8–26% for precision (%RSD). Recovery rates ranged from 66% to 84% for barbiturates, 36% to 110% for benzodiazepines, 41% to 86% for tri/tetracyclic antidepressants, 15% to 81% for drugs of abuse, 28% to 44% for phenethylamines, and 25% to 118% for pesticides. The validated results were used to develop a user-friendly, systematic, and quantitative toxicological GC/MS/MS system and software "Quick-DB Forensic".