Blimp-1 is involved in B cell activation and maturation in Nile tilapia (Oreochromis niloticus). (September 2019)

• Record Type: Journal Article
• Authors: Wu, Liting; Zhou, Enxu; Gao, Along; Kong, Linghe; Wu, Siwei; Bian, Xia; Li, Yuan; Li, Bingxi; Fu, Shengli; Guo, Zheng; Ye, Jianmin
• Is Part Of: Developmental and comparative immunology. Volume 98(2019)
• Page Start: 137
• Page End: 147
• Publication Date: 2019-09
• Subjects: Oreochromis niloticus -- Blimp-1 -- IgM+ B cell -- sIgM -- B cell activation; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2019.05.002
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: B lymphocyte-induced maturation protein 1 (Blimp-1), a transcription factor containing zinc finger, is required and sufficient to trigger terminal differentiation of B cells in mammals. The Blimp-1 ( OnBlimp-1 ) from Nile tilapia ( Oreochromis niloticus ) was identified and characterized its expression pattern during B cell activation and maturation. The cDNA of OnBlimp-1 open reading frame is 2547 bp encoding a protein of 848 amino acids and the predicted molecular weight is 93.36 kDa. OnBlimp-1 contains a SET domain and five ZnF_C2H2 domains, which shares high homology with that of other species. OnBlimp-1 transcription was detected in all examined tissues with high expression in the spleen (SPL). Analysis of sorted lymphocyte populations, including IgM + and IgM − cells from peripheral blood (PBL), SPL and anterior kidney (AK), indicated that the OnBlimp-1 transcription was highly expressed in the IgM + B cells. Upon LPS stimulation, OnBlimp-1 expression was up-regulated in tissues of PBL, SPL and AK significantly. The expression of OnBlimp-1, as well as the secreted IgM, was significantly up-regulated in the SPL and AK leukocytes stimulated with anti-OnIgM monoclonal antibody and LPS in vitro, respectively. Above results suggest that OnBlimp-1, a cytokine regulating the terminal differentiation of activated B cells to antibody-secreting cells, is likely to play important roles in B cell activation and maturation in Nile tilapia. Highlights: Blimp-1 ( OnBlimp-1 ) gene was cloned and identified in Nile tilapia ( Oreochromis niloticus ). OnBlimp-1 expressed at high levels in the spleen tissue as well as IgM + B lymphocytes. LPS stimulation increased OnBlimp-1 expressions in peripheral blood, spleen and anterior kidney in vivo. OnBlimp-1 and sIgM expressions in spleen and anterior kidney leukocytes were up-regulated with LPS stimulation in vitro.