Correlating blood levels of 8‐hydroxydeoxyguanosine to hOGG1 genotypes and the incidence of ischemic cardiomyopathy. Issue 5 (2nd June 2016)

• Record Type: Journal Article
• Authors: Jin, Yu; Qiu, Chun‐Guang; Zheng, Qiang‐Sun; Ding, Shi‐Fang; Jiang, Qi‐Jun
• Is Part Of: Kaohsiung journal of medical sciences. Volume 32:Issue 5(2016)
• Page Start: 241
• Page End: 247
• Publication Date: 2016-06-02
• Subjects: 8‐hydroxy guanine glycosylase -- 8‐hydroxy‐deoxyguanosine -- Ischemic cardiomyopathy -- Oxidative stress -- Polymorphism; Medicine -- Periodicals; 610.5
• DOI: 10.1016/j.kjms.2016.04.010
• Languages: English
• ISSNs: 1607-551X

Abstract

Abstract: We measured the serum levels of 8‐hydroxydeoxyguanosine (8‐OHdG) and investigated whether these levels correlate with incidence of ischemic cardiomyopathy (ICM), and whether these levels correlate with underlying oxidative stress in patients with ICM. Polymerase chain reaction‐restriction fragment length polymorphism analysis was performed to assess the prevalence of the Ser/Cys polymorphism in the human 8‐oxoguanine glycosylase ( hOGG1 ) gene. We analyzed the samples from 246 ICM cases (the ICM group) and another 246 age‐ and sex‐matched volunteers with normal coronary artery function (the control group). Levels of 8‐OHdG in participants' blood samples were 6.7 ± 1.7 and 3.0 ± 0.8 in the ICM and control groups, respectively ( p < 0.05). Although there were no differences in allele frequency ( p = 0.140), significant differences were present in the genotype distributions ( p = 0.002). The Cys/Cys genotype correlated strongly with the risk of developing ICM (odds ratio, 2.2; 95% confidence interval, 1.4–3.3). Treating the Ser/Ser and Ser/Cys genotypes as members of the same group increased the predicted ICM risk for patients carrying the Cys/Cys genotype (odds ratio, 1.9; 95% confidence interval, 1.2–2.9). The serum level of 8‐OHdG in the ICM group was higher than that in the control group ( p < 0.05) and significantly increased in those carrying the Cys/Cys genotype (8.7 ± 1.7 for the Cys/Cys group, and 4.5 ± 0.8 for the Ser/Ser+Ser/Cys group; p < 0.05). Patients carrying the Cys/Cys genotype had a significantly increased risk of developing ICM. Serum levels of 8‐OHdG were significantly increased in patients with ICM.