Glycine‐modified growth hormone secretagogues identified in seized doping material. Issue 2 (11th September 2018)

• Record Type: Journal Article
• Authors: Gajda, Paulina Marta; Holm, Niels Bjerre; Hoej, Lars Jakobsen; Rasmussen, Brian Schou; Dalsgaard, Petur Weihe; Reitzel, Lotte Ask; Linnet, Kristian
• Is Part Of: Drug testing and analysis. Volume 11:Issue 2(2019)
• Page Start: 350
• Page End: 354
• Publication Date: 2018-09-11
• Subjects: doping -- growth hormone secretagogues -- LC–MS -- modified GRF 1–29 -- Peptide hormones; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.2489
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract: A number of unknown pharmaceutical preparations seized by Danish customs authorities were submitted for liquid chromatography–high resolution mass spectrometry (LC–HRMS) analysis. Comparison with reference standards unequivocally identified the content of the powders as analogs of the growth hormone secretagogues GHRP‐2 (Pralmorelin), GHRP‐6, Ipamorelin, and modified growth hormone releasing factor (modified GRF 1–29), which can be used as performance‐enhancing substances in sports. In all cases, the detected modification involved the addition of an extra glycine amino acid at the N ‐terminus, and analytical methods targeting growth hormone secretagogues should hence be updated accordingly. Abstract : A number of unknown pharmaceutical preparations seized by Danish customs authorities were submitted for liquid chromatography high‐resolution mass spectrometry (LC‐HR‐MS) analysis. Comparison with reference standards unequivocally identified the content of the powders as analogs of the growth hormone secretagogues GHRP‐2 (Pralmorelin), GHRP‐6, Ipamorelin and modified growth hormone releasing factor (modified GRF 1‐29), which can be used as performance‐enhancing substances in sports. In all cases, the detected modification involved the addition of an extra glycine amino acid at the N‐terminus, and analytical methods targeting growth hormone secretagogues should hence be updated accordingly.