Isolation and Determination of Beta-Carotene in Carrots by Magnetic Chitosan Beta-Cyclodextrin Extraction and High-Performance Liquid Chromatography (HPLC). (24th July 2019)

• Record Type: Journal Article
• Authors: Dai, Yunliang; Row, Kyung Ho
• Is Part Of: Analytical letters. Volume 52:Number 11(2019)
• Page Start: 1828
• Page End: 1843
• Publication Date: 2019-07-24
• Subjects: Beta-carotene -- Fourier transform-infrared (FT-IR) spectroscopy -- high-performance liquid chromatography (HPLC) -- magnetic chitosan beta-cyclodextrin biopolymers -- thermogravimetric analysis (TGA); Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2019.1570245
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Beta-carotene is a carotenoid with strong antioxidant activity that has been used in many areas and attracted significant attention. Among the articles reported, however, there are few examples of the convenient and eco-friendly use of magnetic chitosan biopolymers to extract beta-carotene. This paper reports the ultrasonic-assisted solid phase extraction of beta-carotene. The beta-carotene in carrot samples was extracted using synthesized magnetic chitosan beta-cyclodextrin biopolymers prior to high-performance liquid chromatography (HPLC) detection. Under the optimal conditions, the beta-carotene content of carrot was found to be 41.06 ± 0.02 μg/g. This newly developed method using magnetic chitosan cyclodextrin biopolymers is a promising method for the ultrasonic-assisted solid phase extraction of carotenoids from vegetables because the procedure is facile and rapid and the magnetic biopolymers can be removed using simple process.