Expression Profile of IL-35 mRNA in Gingiva of Chronic Periodontitis and Aggressive Periodontitis Patients: A Semiquantitative RT-PCR Study. Issue 6 (27th November 2013)

• Record Type: Journal Article
• Authors: Kalburgi, Nagaraj B.; Muley, Akshay; Shivaprasad, B. M.; Koregol, Arati C.
• Other Names: Chace Donald H. Academic Editor.
• Is Part Of: Disease markers. Volume 35:Issue 6(2013)
• Page Start: 819
• Page End: 823
• Publication Date: 2013-11-27
• Subjects: Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.1155/2013/489648
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : Background . Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells (T r e g s ) and is required for their maximal suppressive activity.T r e g s are involved in the modulation of local immune response in chronic periodontitis patients. Objective . Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients. Materials and Methods . The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Results . The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects (6.87 ± 2.32 ) as compared to the aggressive periodontitis group (4.71 ± 1.43 ) and least seen in healthy patients (3.03 ± 1.91 ). Conclusion . The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.