A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites. (14th February 2017)

• Record Type: Journal Article
• Authors: Defaus, Sira; Gallo, Maria; Abengózar, María A.; Rivas, Luis; Andreu, David
• Other Names: Wade John D. Academic Editor.
• Is Part Of: International journal of peptides. Volume 2017(2017)
• Publication Date: 2017-02-14
• Subjects: Peptides -- Periodicals; Peptides; Peptides; Electronic journals; Periodical; Periodicals; Periodicals; Internet Resources; Fulltext
• DOI: 10.1155/2017/4213037
• Languages: English
• ISSNs: 1687-9767

Abstract

Abstract : A successful approach to deliver paromomycin, a poorly absorbed aminoglycoside antibiotic, to parasite cells is reported, based on selective protection of amino and hydroxyl groups followed by conjugation to a fluorolabeled, PEG-functionalized cell-penetrating Tat(48-60) peptide. The resulting construct is efficiently internalized into Leishmania cells, evidencing the fitness of cell-penetrating peptides as vectors for efficiently transporting low-bioavailability drugs into cells.