Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase. (22nd February 2009)

• Record Type: Journal Article
• Authors: Basso, Alessandra; Sinigoi, Loris; Gardossi, Lucia; Flitsch, Sabine
• Other Names: El-Faham Ayman Academic Editor.
• Is Part Of: International journal of peptides. Volume 2009(2009)
• Publication Date: 2009-02-22
• Subjects: Peptides -- Periodicals; Peptides; Peptides; Electronic journals; Periodical; Periodicals; Periodicals; Internet Resources; Fulltext
• DOI: 10.1155/2009/362482
• Languages: English
• ISSNs: 1687-9767

Abstract

Abstract : Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase peptide synthesis when both chemical and biocatalytic strategies are used.