A Biocompatible Nanocomposite for Glucose Sensing. (10th April 2011)

• Record Type: Journal Article
• Authors: Rahimi, Parvaneh; Ghourchian, Hedayatollah; Rafiee-Pour, Hossain-Ali; Norouzi, Parviz; Ganjali, Mohammad Reza
• Other Names: Ozkan Sibel A. Academic Editor.
• Is Part Of: International journal of electrochemistry. Volume 2011(2011)
• Publication Date: 2011-04-10
• Subjects: Electrochemistry -- Periodicals; Electrochemistry; Periodicals; 660.297
• DOI: 10.4061/2011/470607
• Languages: English
• ISSNs: 2090-3529

Abstract

Abstract : A nanocomposite containing amine functionalized multiwalled carbon nanotubes and a room temperature ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate) was prepared and applied for glucose oxidase (GOx) immobilization on glassy carbon electrode. The proposed nanocomposite provided a favorable microenvironment to preserve the bioactivity of GOx. It could also effectively facilitate the enzyme direct electron transfer to the electrode. This brought about a remarkable improvement in the sensitivity of the glucose biosensor. Under the optimum experimental conditions, the formal potential of GOx was about −467.5 mV. Moreover, the sensitivity and response time of the biosensor toward glucose were 1277 μ A mM -1 cm -2 and 6 s, respectively. The biosensor provided a linear dynamic response between 0.1 and 43 μ M with a very low detection limit of 63 nM. Also, the values for apparent Michaelis-Menten constant and maximum current were obtained as 18 μ M and 2.7 μ A, respectively.