Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules. (21st January 2010)

• Record Type: Journal Article
• Authors: Zeng, Songjun; Liu, Hongrong; Yang, Qibin
• Other Names: Zhao Shan Academic Editor.
• Is Part Of: International journal of biomedical imaging. Volume 2010(2010)
• Publication Date: 2010-01-21
• Subjects: Diagnostic imaging -- Periodicals; Imaging systems in medicine -- Periodicals; Imagerie pour le diagnostic; Imagerie médicale; Diagnostic imaging; Imaging systems in medicine; Diagnostic Imaging -- Periodicals; Electronic journals; Periodicals; 616.0754
• DOI: 10.1155/2010/195274
• Languages: English
• ISSNs: 1687-4188

Abstract

Abstract : A method for three-dimensional (3D) reconstruction of macromolecule assembles, that is, octahedral symmetrical adapted functions (OSAFs) method, was introduced in this paper and a series of formulations for reconstruction by OSAF method were derived. To verify the feasibility and advantages of the method, two octahedral symmetrical macromolecules, that is, heat shock proteinDegp 24 and the Red-cell L Ferritin, were utilized as examples to implement reconstruction by the OSAF method. The schedule for simulation was designed as follows: 2000 random orientated projections of single particles with predefined Euler angles and centers of origins were generated, then different levels of noises that is signal-to-noise ratio (S/N)= 0.1, 0.5, and 0.8 were added. The structures reconstructed by the OSAF method were in good agreement with the standard models and the relative errors of the structures reconstructed by the OSAF method to standard structures were very little even for high level noise. The facts mentioned above account for that the OSAF method is feasible and efficient approach to reconstruct structures of macromolecules and have ability to suppress the influence of noise.