Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility. (30th October 2016)

• Record Type: Journal Article
• Authors: Faro, Jonathan; Mitchell, Malika; Chen, Yuh-Jue; Kamal, Sarah; Riddle, Gerald; Faro, Sebastian
• Other Names: Larsen Bryan Academic Editor.
• Is Part Of: Infectious diseases in obstetrics and gynecology. Volume 2016(2016)
• Publication Date: 2016-10-30
• Subjects: Generative organs, Female -- Infections -- Periodicals; Communicable diseases in pregnancy -- Periodicals; 618.142005
• DOI: 10.1155/2016/5293034
• Languages: English
• ISSNs: 1064-7449

Abstract

Abstract : Background . Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods . Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings . Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 10 5 bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus ). Sensitivity was increased to 10 −2 bacteria/mL for GBS, 10 −1 bacteria/mL for E. faecalis, and 10 1 bacteria/mL for N. gonorrhoeae following 18–24-hour culture. Conclusion . This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.