The genome sequence of the soft-rot fungus Penicillium purpurogenum reveals a high gene dosage for lignocellulolytic enzymes. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: Mardones, Wladimir; Di Genova, Alex; Cortés, María Paz; Travisany, Dante; Maass, Alejandro; Eyzaguirre, Jaime
• Is Part Of: Mycology. Volume 9:Issue 1(2018)
• Page Start: 59
• Page End: 69
• Publication Date: 2018-01-02
• Subjects: Penicillium purpurogenum -- genome sequencing -- lignocellulose biodegradation -- CAZymes -- RNA-seq -- Illumina; Fungi -- Periodicals; Mycology -- Periodicals; Fungi; Mycology; Periodicals; 579.505
• DOI: 10.1080/21501203.2017.1419995
• Languages: English
• ISSNs: 2150-1203

Abstract

ABSTRACT: The high lignocellulolytic activity displayed by the soft-rot fungus Penicillium purpurogenum has made it a target for the study of novel lignocellulolytic enzymes. We have obtained a reference genome of 36.2 Mb of non-redundant sequence (11, 057 protein-coding genes). The 49 largest scaffolds cover 90% of the assembly, and Core Eukaryotic Genes Mapping Approach (CEGMA) analysis reveals that our assembly captures almost all protein-coding genes. RNA-seq was performed and 93.1% of the reads aligned to the assembled genome. These data, plus the independent sequencing of a set of genes of lignocellulose-degrading enzymes, validate the quality of the genome sequence. P. purpurogenum shows a higher number of proteins with CAZy motifs, transcription factors and transporters as compared to other sequenced Penicillia . These results demonstrate the great potential for lignocellulolytic activity of this fungus and the possible use of its enzymes in related industrial applications.