Plasticity of oral mucosal cell sheets for accelerated and scarless skin wound healing. (December 2017)

• Record Type: Journal Article
• Authors: Roh, Jong-Lyel; Lee, Jaewang; Kim, Eun Hye; Shin, Daiha
• Is Part Of: Oral oncology. Volume 75(2017)
• Page Start: 81
• Page End: 88
• Publication Date: 2017-12
• Subjects: Skin -- Wound healing -- Cell sheet -- Oral mucosa -- Graft -- Plasticity; Mouth -- Cancer -- Periodicals; Mouth -- Tumors -- Periodicals; Mouth Diseases -- Periodicals; Mouth Neoplasms -- Periodicals; Bouche -- Cancer -- Périodiques; Bouche -- Tumeurs -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9943105
• DOI: 10.1016/j.oraloncology.2017.10.024
• Languages: English
• ISSNs: 1368-8375

Abstract

Highlights: Keratinocytes and fibroblasts derived from the oral mucosa proliferated faster than those derived from the skin. The in vitro- engineered mucosa equivalent was developed using autologous mucosa and fibrin. The mucosal and skin cell sheets had similar histological structures to oral mucosa or skin. The mucosal and skin cell sheet promoted skin wound healing with early closure and less scarring. The oral mucosal cell sheet showed in vivo tissue plasticity in cutaneous wounds. Abstract: Objectives: Wound healing is generally faster and associated with less scarring in the oral mucosa than in the skin. Although rarely studied, oral mucosa equivalents may contribute to rapid, scarless cutaneous wound healing. Therefore, we examined the potential utility of our newly developed oral mucosal cell sheet in skin wound healing. Materials and methods: Oral mucosa and skin samples were obtained from surgical patients and Sprague-Dawley rats. Keratinocytes and fibroblasts were primarily cultured for in vitro cell expansion. Mucosa and skin equivalents were produced with a mixture of cultured fibroblasts and autologous fibrin from plasma and seeding keratinocytes. Mucosal and skin cell sheets were transplanted in full-thickness excisional wounds of rat skin with control wounds. Gross, histological, and molecular characteristics of wound healing according to different postsurgical days were compared in control and cell sheet-covered wounds. Results: Keratinocytes and fibroblasts derived from the oral mucosa were cultured faster than those derived from the skin. The in vitro -engineered oral mucosa and skin equivalents were successfully produced using complete autologous mucosa or skin and plasma fibrin, showing similarity to the histological characteristics of the skin or mucosa. In the in vivo rat model, the oral mucosal and skin cell sheet promoted wound healing with early wound closure and less scarring. The cell sheet-treated wounds showed lower TGF-β1, α-smooth muscle actin, and fibronectin mRNA expression than the control wounds. Conclusions: The oral mucosal cell sheet demonstrated in vivo tissue plasticity through good adaptation to skin wounds, contributing to accelerated and scarless healing. Condensed abstract: This study examined the potential utility of a newly developed oral mucosal cell sheet in skin excisional wounds. The oral mucosal cell sheet promoted wound healing with early wound closure and less scarring and demonstrated in vivo tissue plasticity through good adaptation in cutaneous wounds.