HD-03/ES: A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in Transfected Human Hepatocarcinoma PLC/PRF/5 Cells. (10th April 2013)

• Record Type: Journal Article
• Authors: Varma, Sandeep R.; Sundaram, R.; Gopumadhavan, S.; Vidyashankar, Satyakumar; Patki, Pralhad S.
• Other Names: Hiasa Yoichi Academic Editor.
• Is Part Of: Hepatitis research and treatment. Volume 2013(2013)
• Publication Date: 2013-04-10
• Subjects: Hepatitis -- Periodicals; Hepatitis; Hepatitis; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 616.3623
• DOI: 10.1155/2013/125398
• Languages: English
• ISSNs: 2090-1364

Abstract

Abstract : HD-03/ES is a herbal formulation used for the treatment of hepatitis B. However, the molecular mechanism involved in the antihepatitis B (HBV) activity of this drug has not been studied using in vitro models. The effect of HD-03/ES on hepatitis B surface antigen (HBsAg) secretion and its gene expression was studied in transfected human hepatocarcinoma PLC/PRF/5 cells. The anti-HBV activity was tested based on the inhibition of HBsAg secretion into the culture media, as detected by HBsAg-specific antibody-mediated enzyme assay (ELISA) at concentrations ranging from 125 to 1000 μ g/mL. The effect of HD-03/ES on HBsAg gene expression was analyzed using semiquantitative multiplex RT-PCR by employing specific primers. The results showed that HD-03/ES suppressed HBsAg production with an IC50 of 380 μ g/mL in PLC/PRF/5 cells for a period of 24 h. HD-03/ES downregulated HBsAg gene expression in PLC/PRF/5 cells. In conclusion, HD-03/ES exhibits strong anti-HBV properties by inhibiting the secretion of hepatitis B surface antigen in PLC/PRF/5 cells, and this action is targeted at the transcription level. Thus, HD-03/ES could be beneficial in the treatment of acute and chronic hepatitis B infections.