CD56bright NK IL‐7Rα expression negatively associates with HCV level, and IL‐7‐induced NK function is impaired during HCV and HIV infections. Issue 1 (11th April 2017) - Judge, Chelsey J.
Abstract : IL‐7‐dependent NK cell activation and effector function to be impaired in viral infections. Abstract : Several lines of evidence support the concept that NK cells play an important role in control of hepatitis C virus (HCV) infection via cytokine secretion and cytotoxicity. IL‐7 is a homeostatic cytokine with a role in T cell development, activation, proliferation, and cytokine secretion. The IL‐7Rα chain [cluster of differentiation (CD)127] is expressed on NK cells, with greatest abundance on the CD56 bright CD16 dim/− (CD56 bright ) subset. Here, we measured CD127 expression on CD56 bright, CD56 dim CD16 + (CD56 dim ), or CD56 neg CD16 + (CD56 neg ) NK cell subsets of 25 uninfected donors (UD); 34 chronic HCV‐infected, treatment‐naiüve; 25 HIV‐infected, virally suppressed on antiretroviral therapy (ART); and 42 HCV–HIV‐coinfected subjects on ART. Interestingly, CD127 expression on CD56 bright NK cells negatively correlated with HCV plasma levels in HCV monoinfection and HCV–HIV coinfection. IL‐7 induced CD69 expression, as well as IFN‐γ production, in CD56 bright NK cells and also enhanced the IFN‐α‐induced CD69 expression on these cells. The latter was impaired in HIV infection. Furthermore, IL‐7 induced B cell lymphoma 2 (BCL‐2) expression and cell cycling of CD56 bright NK cells, and this effect was impaired in HCV‐ and HIV‐infected subjects. Whereas IL‐7‐stimulated CD56 bright NK cell degranulation appeared intact in all cohorts, we observed impaired IL‐7‐activated NK cell cytolytic function in HCV‐ and HIV‐infected subjects. Finally, IL‐7‐induced phosphorylation of STAT‐5 (pSTAT‐5) signaling was impaired in NK cells of subjects with chronic viral infection, and this was reversible upon 6 mo of viral suppression with IFN‐free HCV therapy. These results implicate that IL‐7‐dependent NK cell activation and effector function may be other host immune surveillance mechanisms that are impaired in viral infections.