Preparation of carbon dot‐based ratiometric fluorescent probes for cellular imaging from Curcuma longa. (18th July 2017)

• Record Type: Journal Article
• Authors: Mazrad, Zihnil Adha Islamy; Kang, Eun Bi; In, Insik; Park, Sung Young
• Is Part Of: Luminescence. Volume 33:Number 1(2018)
• Page Start: 40
• Page End: 46
• Publication Date: 2017-07-18
• Subjects: biocompatible -- bioimaging -- carbon dots -- Curcuma longa; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.3370
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: This work derived biocompatible and stable probes based on fluorescent nanoparticles (FNPs) from a natural source, Curcuma longa . The multi‐color fluorescence emissions from carbonized Curcuma longa (C‐FNPs) obtained through defined dehydration conditions are soluble in water and have a small particle size (~17 nm). The surface passivation with polyethylene glycol (PEG) capped with amine groups in FNPs (P‐FNPs) generated a probe with a higher quantum yield and longer fluorescence lifetime than obtained with C‐FNPs. The X‐ray photoelectron spectroscopy and X‐ray diffraction spectra confirmed the associated chemical moieties of C‐FNPs and P‐FNPs. Furthermore, the prepared material showed non‐toxic effects with almost 100% cell viability, even at high concentrations. In conclusion, fluorescence sensors from natural sources may be useful for numerous biomedical research applications.