Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. Issue 15 (1st June 2015)

• Record Type: Journal Article
• Authors: Dojnov, Biljana; Grujić, Marica; Vujčić, Zoran
• Other Names: Lapizco‐Encinas B. H. guestEditor.; Hayes M. A. guestEditor.
• Is Part Of: Electrophoresis. Volume 36:Issue 15(2015)
• Page Start: 1724
• Page End: 1727
• Publication Date: 2015-06-01
• Subjects: β‐Glucosidase -- Cellulase -- Endocellulase -- Exocellulase -- SSF -- Trichoderma -- Zymogram; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201400541
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4‐methylumbelliferyl‐β‐d ‐cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.