Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats. (24th May 2016)

• Record Type: Journal Article
• Authors: Nobre, Maria Elizabeth Pereira; Correia, Alyne Oliveira; Mendonça, Francisco Nilson Maciel; Uchoa, Luiz Ricardo Araújo; Vasconcelos, Jessica Tamara Nunes; de Araújo, Carlos Ney Alencar; Brito, Gerly Anne de Castro; Siqueira, Rafaelly Maria Pinheiro; Cerqueira, Gilberto dos Santos; Neves, Kelly Rose Tavares; Arida, Ricardo Mário; Viana, Glauce Socorro de Barros
• Other Names: Li Duo Academic Editor.
• Is Part Of: Journal of nutrition and metabolism. Volume 2016(2016)
• Publication Date: 2016-05-24
• Subjects: Nutrition -- Periodicals; Metabolism -- Periodicals; Diet in disease -- Periodicals; Metabolic Diseases; Metabolism; Nutrition Disorders; Nutritional Sciences; Diet in disease; Metabolism; Nutrition; Electronic journals; Periodicals; Periodicals; 363.8
• DOI: 10.1155/2016/6462120
• Languages: English
• ISSNs: 2090-0724

Abstract

Abstract : Background . Omega-3 (ω 3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects ofω 3, in a model of global ischemia. Methods . Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups were SO, untreated ischemic and ischemic treated rats withω 3 (5 and 10 mg/kg, 7 days). The SO and untreated ischemic animals were orally treated with 1% cremophor and, 1 h after the last administration, they were behaviorally tested and euthanized for neurochemical (DA, DOPAC, and NE determinations), histological (Fluoro jade staining), and immunohistochemical (TNF-alpha, COX-2 and iNOS) evaluations. The data were analyzed by ANOVA and Newman-Keuls as the post hoc test. Results . Ischemia increased the locomotor activity and rearing behavior that were partly reversed byω 3. Ischemia decreased striatal DA and DOPAC contents and increased NE contents, effects reversed byω 3. This drug protected hippocampal neuron degeneration, as observed by Fluoro-Jade staining, and the increased immunostainings for TNF-alpha, COX-2, and iNOS were partly or totally blocked byω 3. Conclusion . This study showed a neuroprotective effect ofω 3, in great part due to its anti-inflammatory properties, stimulating translational studies focusing on its use in clinic for stroke managing.