Rapid degradation of long-chain crude oil in soil by indigenous bacteria using fermented food waste supernatant. (15th February 2019)

• Record Type: Journal Article
• Authors: Xu, Jinlan; Zhang, Qiuju; Li, Dongyuan; Du, Juan; Wang, Cong; Qin, Jinyi
• Is Part Of: Waste management. Volume 85(2019)
• Page Start: 361
• Page End: 373
• Publication Date: 2019-02-15
• Subjects: Rapid bioremediation -- Long-chain alkanes -- Food waste -- Fermented supernatant -- Indigenous bacteria -- Oil-contaminated soil; TPH total petroleum hydrocarbons -- FS fermented food waste supernatant -- IHD indigenous hydrocarbon degraders -- NH4+-N ammonia nitrogen -- NO3¯-N nitrate nitrogen -- NO2¯-N nitrite nitrogen -- C/N molar ratio of consumed carbon (contained in glucose and acetic acid) to consumed ammonia nitrogen -- YPD yeast extract peptone dextrose -- DOC dissolved organic carbon -- FID flame ionization detector -- GC gas chromatograph -- TC total carbon -- IC inorganic carbon -- ICP-MS inductively coupled plasma mass spectrometry -- TCD thermal conductivity detector -- VFA volatile fatty acids -- SD standard deviation -- OS the analysis result of microbial diversity in original crude oil-contaminated soil S2 before adding FS -- FSS the analysis result of microbial diversity in soil S2 after adding FS in 30 days of incubation -- FSL the analysis result of microbial diversity of cultivable hydrocarbon degraders in soil S2 after adding FS in 30 days of incubation; Hazardous wastes -- Periodicals; Refuse and refuse disposal -- Periodicals; 363.728
• DOI: 10.1016/j.wasman.2018.12.041
• Languages: English
• ISSNs: 0956-053X

Abstract

Graphical abstract: The improvement mechanism of rapid degradation long-chain alkanes in soil by addition of fermented food waste supernatant. Highlights: Long-chain alkanes were main carbon sources for IHD after 14 days. Long-chain alkanes were biodegraded rapidly by addition of fermented supernatant. The glucose and acetic acid in fermented supernatant accelerated NH4 + -N consumption. The considerable consumed C/N promoted the grow of Acinetobacter and Aquabacterium . The genus Aquabacterium which was uncultivable became the dominant bacteria. Abstract: The objective of this study is to explore how to stimulate soil indigenous bacteria for the degradation of long-chain crude oil by adding fermented food waste supernatant (FS). Four concentrations of FS (0 mL, 0.1 mL, 1 mL, and 3 mL) were added to two oil-contaminated soils S1 and S2 for 30 days of bioremediation experiments. The results showed that the biodegradation of long-chain alkanes (C29 - C24 ) could reach up to 1756 mg/kg (49.3%, S1) and 3937 mg/kg (43.9%, S2), which were 3.1 and 3.2 times that of the non-nutrient system. In addition, the logarithmic growth rate of the indigenous hydrocarbon degraders (IHD) reached 41.5%. The long-chain crude oil can be rapidly degraded by indigenous bacteria with FS added in a short time. The glucose and acetic acid accelerated the consumption of ammonia nitrogen (NH4 + -N) in the prophase of bioremediation and the molar ratio of consumed carbon (contained in glucose and acetic acid) to consumed NH4 + -N (C/N) was high by adding FS. Thus, the IHD can multiply rapidly. The analysis of microbial diversity revealed that the IHD (genera Acinetobacter and Aquabacterium ) became the dominant bacteria. Long-chain alkanes became the main carbon sources for IHD after 14 days in soil S1 and 16 days in soil S2. Thus, the rapid biodegradation of long-chain crude oil was achieved. The genus Aquabacterium which was uncultivable on crude oil medium became the dominant bacteria. This study provides an environment-friendly and sustainable remediation technology for bioremediation of oil-contaminated soils.