Comparative Neuroprotective Effects of Dietary Curcumin and Solid Lipid Curcumin Particles in Cultured Mouse Neuroblastoma Cells after Exposure to Aβ42. (16th April 2017)

• Record Type: Journal Article
• Authors: Maiti, Panchanan; Dunbar, Gary L.
• Other Names: Kuret Jeff Academic Editor.
• Is Part Of: International journal of alzheimer's disease. Volume 2017(2017)
• Publication Date: 2017-04-16
• Subjects: Alzheimer's disease -- Periodicals; 616.831005
• DOI: 10.1155/2017/4164872
• Languages: English
• ISSNs: 2090-8024

Abstract

Abstract : Aggregation of amyloid beta protein (A β ) and phosphorylated tau (p-Tau) plays critical roles in pathogenesis of Alzheimer's disease (AD). As an antiamyloid natural polyphenol, curcumin (Cur) has a potential role in prevention of neurodegeneration in AD. However, due to limited absorption of the dietary Cur, the solid lipid Cur particles (SLCP) have been suggested as being more effective for AD therapy. In the present study, we compared the role of dietary Cur and SLCP on oxidative stress, neuronal death, p-Tau level, and certain cell survival markers in vitro, after exposure to A β 42. Mouse neuroblastoma cells were exposed to A β 42 for 24 h and incubated with or without dietary Cur and/or SLCP. Reactive oxygen species (ROS), apoptotic cell death, p-Tau, and tau kinase (including GSK-3 β and cell survival markers, such as total Akt, phosphorylated Akt, and PSD95 levels) were investigated. SLCP showed greater permeability than dietary Cur in vitro, decreased ROS production, and prevented apoptotic death. In addition, SLCP also inhibited p-Tau formation and significantly decreased GSK-3 β levels. Further, the cell survival markers, such as total Akt, p-Akt, and PSD95 levels, were more effectively maintained by SLCP than dietary Cur in A β 42 exposed cells. Therefore, SLCP may provide greater neuroprotection than dietary Cur in Alzheimer's disease.