Pdx1 and controlled culture conditions induced differentiation of human amniotic fluid‐derived stem cells to insulin‐producing clusters. (13th November 2012)

• Record Type: Journal Article
• Authors: Chun, So Young; Mack, David L.; Moorefield, Emily; Oh, Se Heang; Kwon, Tae Gyun; Pettenati, Mark J.; Yoo, James J.; Coppi, Paolo De; Atala, Anthony; Soker, Shay
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 9:Number 5(2015:May)
• Page Start: 540
• Page End: 549
• Publication Date: 2012-11-13
• Subjects: amniotic fluid‐derived stem cells -- Pdx1 -- differentiation -- diabetes -- cell therapy -- growth factors -- extracellular matrix components; Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.1631
• Languages: English
• ISSNs: 1932-6254

Abstract

Abstract: This study investigated the differentiation of human amniotic fluid‐derived stem cells (hAFSCs) into insulin‐producing clusters in vitro . Adenovirally‐delivered mouse Pdx1 (Ad‐ Pdx1 ) induced human Pdx1 expression in hAFSCs and enhanced the coordinated expression of downstream β ‐cell markers. When Ad‐ Pdx1 ‐transduced hAFSCs were sequentially treated with activin A, bFGF and nicotinamide and the culture plate surface coated with poly‐l ‐ornithine, the expression of islet‐associated human mRNAs for Pdx1, Pax6, Ngn3 and insulin was increased. C‐peptide ELISA confirmed that Ad‐ Pdx1 ‐transduced hAFSCs processed and secreted insulin in a manner consistent with that pathway in pancreatic β ‐cells. To sustain the β ‐cell‐like phenotype and investigate the effect of three‐dimensional (3D) conformation on the differentiation of hAFSCs, Pdx1 ‐transduced cells were encapsulated in alginate and cultured long‐term under serum‐free conditions. Over 2 weeks, partially differentiated hAFSC clusters increased in size and increased insulin secretion. Taken together, these data demonstrate that ectopic Pdx1 expression initiates pancreatic differentiation in hAFSCs and that a β ‐cell‐like phenotype can be augmented by culture conditions that mimic the stromal components and 3D geometry associated with pancreatic islets. Copyright © 2012 John Wiley & Sons, Ltd.