Evaluation of the new AspID polymerase chain reaction assay for detection of Aspergillus species: A pilot study. Issue 6 (23rd March 2018)

• Record Type: Journal Article
• Authors: Prattes, Juergen; Hoenigl, Martin; Zinke, Stefanie E.‐M.; Heldt, Sven; Eigl, Susanne; Johnson, Gemma L.; Bustin, Stephen; Stelzl, Evelyn; Kessler, Harald H.
• Is Part Of: Mycoses. Volume 61:Issue 6(2018)
• Page Start: 355
• Page End: 359
• Publication Date: 2018-03-23
• Subjects: Asp ID -- BALF -- galactomannan -- invasive aspergillosis -- PCR; Pathogenic fungi -- Periodicals; Medical mycology -- Periodicals; 616.969
• DOI: 10.1111/myc.12757
• Languages: English
• ISSNs: 0933-7407

Abstract

Summary: The newly developed Asp ID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis (IPA) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the Asp ID assay were 94.1% (95% CI 73.3‐99.9), 76.5% (95% CI 50.1‐93.2), 4 (95% CI 1.7‐9.5) and 0.1 (95% CI 0.01‐0.5) respectively.