Spectrophotometric Method for Determination of Five 1, 4-Dihydropyridine Drugs Using N-Bromosuccinimide and Indigo Carmine Dye. (22nd August 2013)

• Record Type: Journal Article
• Authors: El Hamd, Mohamed A.; Derayea, Sayed M.; Abdelmageed, Osama H.; Askal, Hassan F.
• Other Names: Arslan Hakan Academic Editor.
• Is Part Of: International journal of spectroscopy. Volume 2013(2013)
• Publication Date: 2013-08-22
• Subjects: Spectrum analysis -- Periodicals; Spectrum Analysis; Spectrum analysis; Periodicals; Periodicals; 543.5
• DOI: 10.1155/2013/243059
• Languages: English
• ISSNs: 1687-9449

Abstract

Abstract : Indirect spectrophotometric method is described for quantification of five of 1, 4-dihydropyridine (1, 4-DHP) drugs using N -bromosuccinimide (NBS) with the aid of indigo carmine (INC) dye. The method is based on addition of known excess of NBS to an acidified solution of 1, 4-DHP drugs and determining the residual of NBS through its ability to bleach the colour of the used dye; the amount of NBS that reacted corresponded to the amount of drugs. Beer's law is obeyed in the concentration range 1.25–13.00 μ g/mL. Good correlation coefficients (0.998-0.999) were found between the absorbance values and the corresponding concentrations. Limits of detections ranged from 0.141 to 0.500 μ g/mL. The proposed method was successfully applied to the analysis of dosage forms; percent of recoveries ranged from 97.31 to 99.46% without interference from any common excipients. The statistical comparison by Student's t -test and variance ratio F -test showed no significant difference between the proposed and official or reported methods.