Highly Sensitive Electrochemical Sensor for the Determination of 8-Hydroxy-2′-deoxyguanosine Incorporating SWCNTs-Nafion Composite Film. (13th September 2015)

• Record Type: Journal Article
• Authors: Yang, Lingfei; Wang, Bing; Qi, Honglan; Gao, Qiang; Li, Chen-zhong; Zhang, Chengxiao
• Other Names: Samitier Josep Academic Editor.
• Is Part Of: Journal of sensors. Volume 2015(2015)
• Publication Date: 2015-09-13
• Subjects: Detectors -- Periodicals; 681.205
• DOI: 10.1155/2015/504869
• Languages: English
• ISSNs: 1687-725X

Abstract

Abstract : 8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a typical biomarker of oxidative DNA damage and has attracted much attention in recent years since the level of 8-OHdG in body fluids is typically associated with various diseases. In this work, a simple and highly sensitive electrochemical sensor for the determination of 8-OHdG was fabricated incorporating single wall carbon nanotubes- (SWCNTs-) Nafion composite film coated on glassy carbon electrode. Nafion was chosen as an optimal adhesive agent from a series of adhesive agents and acted as a binder, enrichment, and exclusion film. Due to the strong cation-exchange ability of Nafion and the outstanding electronic properties of SWCNTs, the prepared SWCNTs-Nafion film can strongly enhance the electrochemical response to oxidation of 8-OHdG and efficiently alleviate the interferences from uric acid and ascorbic acid. The oxidation peak currents are linear with the concentration of 8-OHdG in the range of 0.03 to 1.25 μ M with a detection limit of 8.0 nM (S/N = 3). This work demonstrates that SWCNTs-Nafion film can improve the sensitivity, selectivity, reproducibility, and stability, making it an ideal candidate for electrochemical detection of 8-OHdG.