Design and Synthesis of a Dinuclear Copper(II) Probe for Selective Fluorescence Sensing of Pyrophosphate. (28th February 2019)

• Record Type: Journal Article
• Authors: Xu, Jinhe; Li, Jing; Liu, Chenxi; Yang, Linlin; He, Guangjie; Ni, Tianjun; Fan, Aiying; Wang, Songjun; Wang, Qingzhi
• Other Names: Paolesse Roberto Academic Editor.
• Is Part Of: Journal of sensors. Volume 2019(2019)
• Publication Date: 2019-02-28
• Subjects: Detectors -- Periodicals; 681.205
• DOI: 10.1155/2019/5349124
• Languages: English
• ISSNs: 1687-725X

Abstract

Abstract : A novel coumarin-based compound DPAC with two dipicolylamine (DPA) arms as the chelator sites was designed and synthesized. The compound DPAC exhibits a highly selective response to Cu 2+ ions with a distinctly emission-quenching phenomenon. Moreover, the in situ formed complex DPAC-Cu 2+ was used for the detection of pyrophosphate (PPi). The binding manner of probe DPAC-Cu 2+ with PPi in 1 : 1 stoichiometry was supported by the Benesi-Hildebrand fitting, ESI-MS and HPLC analysis. The linear range of PPi concentration was 1-4 μ M, and the detection limit was 0.53 μ M. The competing experiments illustrated that the probe DPAC-Cu 2+ had good sensitivity and selectivity for PPi than other anions, including ATP, ADP, AMP, and Pi in CH3 CN : HEPES (3 : 2, v / v, pH = 7.20 ) buffer. Further, cell fluorescence imaging experiments indicated that the probe DPAC-Cu 2+ had a potential to be used to detect PPi in vivo.