Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium. (29th May 2011)

• Record Type: Journal Article
• Authors: Walker, Tobias; Siegel, Julian; Nolte, Andrea; Hartmann, Silke; Kornberger, Angela; Steger, Volker; Wendel, Hans-Peter
• Other Names: Berkhout Ben Academic Editor.
• Is Part Of: Journal of nucleic acids. Volume 2011(2011)
• Publication Date: 2011-05-29
• Subjects: Nucleic acids -- Periodicals; 572.805
• DOI: 10.4061/2011/694789
• Languages: English
• ISSNs: 2090-0201

Abstract

Abstract : Background . Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells. Methods . Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an inflammatory cytokine. Adhesion molecule expression was determined by FACS-analysis, and reduction of intracellular mRNA was determined by qRT-PCR. Furthermore, the attachment of isolated neutrophils on the endothelial layer was determined after siRNA transfection. Results . In summary, siRNA transfection significantly decreased the percentage positive cells in a single cocktail transfection of each adhesion molecule investigated. Adhering neutrophils were diminished as well. Conclusion . siRNA might be a promising tool for the effective suppression of adhesion molecule expression on pulmonary microvascular cells, potentially minimizing leukocyte-endothelial depending interactions of a pulmonary allograft.