Prognostic Significance of Circulating and Endothelial Progenitor Cell Markers in Type 2 Diabetic Foot. (2nd February 2014)

• Record Type: Journal Article
• Authors: Sambataro, Maria; Seganfreddo, Elena; Canal, Fabio; Furlan, Anna; del Pup, Laura; Niero, Monia; Paccagnella, Agostino; Gherlinzoni, Filippo; dei Tos, Angelo Paolo
• Other Names: Vourliotakis Georgios Academic Editor.
• Is Part Of: International journal of vascular medicine. Volume 2014(2014)
• Publication Date: 2014-02-02
• Subjects: Blood-vessels -- Diseases -- Periodicals; 616.13005
• DOI: 10.1155/2014/589412
• Languages: English
• ISSNs: 2090-2824

Abstract

Abstract : Objective . We studied circulating precursor cells (CPC) in type 2 diabetes mellitus (T2DM) with neuropathic foot lesions with or without critical limb ischemia and relationships between endothelial precursor cells (EPC) and peripheral neuropathy. Methods and Subjects . We measured peripheral blood CD34, CD133, and CD45 markers for CPC and KDR, CD31 markers for EPC by citofluorimetry and systemic neural nociceptor CGRP (calcitonin gene related protein) by ELISA in 8 healthy controls (C) and 62 T2DM patients: 14 with neuropathy (N), 20 with neuropathic foot lesions (N1), and 28 with neuroischemic recent revascularized (N2) foot lesions. Timing of lesions was: acute (until 6 weeks), healed, and not healed. Results . CD34+ and CD133+ were reduced in N, N1, and N2 versus C, and CD34+ were lower in N2 versus N1 (P = 0.03 ). In N2 CD34+KDR+ remain elevated in healed versus chronic lesions and, in N1 CD133+31+ were elevated in acute lesions. CGRP was reduced in N2 and N1 versus C (P < 0.04 versus C26 ± 2 pg/mL). CD34+KDR+ correlated in N2 with oximetry and negatively in N1 with CGRP. Conclusions . CD34+ CPC are reduced in diabetes with advanced complications and diabetic foot. CD34+KDR+ and CD31+133+ EPC differentiation could have a prognostic and therapeutic significance in the healing process of neuropathic and neuroischemic lesions.