Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing. (22nd December 2016)

• Record Type: Journal Article
• Authors: Tangkawsakul, Wanida; Srikhirin, Toemsak; Shinbo, Kazunari; Kato, Keizo; Kaneko, Futao; Baba, Akira
• Other Names: Bonn Günther K. Academic Editor.
• Is Part Of: International journal of analytical chemistry. Volume 2016(2016)
• Publication Date: 2016-12-22
• Subjects: Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1155/2016/1432781
• Languages: English
• ISSNs: 1687-8760

Abstract

Abstract : In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test. We obtained the lowest detection limits of 1.58 × 10 5 cells/ml for RBC-A and 3.83 × 10 5 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 10 8 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing.