Direct Observation of Amyloid β Behavior at Phospholipid Membrane Constructed on Gold Nanoparticles. (2nd December 2018)

• Record Type: Journal Article
• Authors: Suga, Keishi; Lai, Ying-Chen; Faried, Miftah; Umakoshi, Hiroshi
• Other Names: Lubman David M. Academic Editor.
• Is Part Of: International journal of analytical chemistry. Volume 2018(2018)
• Publication Date: 2018-12-02
• Subjects: Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1155/2018/2571808
• Languages: English
• ISSNs: 1687-8760

Abstract

Abstract : Amyloid β (A β ) is a potential biomarker of Alzheimer's disease (AD), and its fibrillation behavior is of interest and value. In this study, the A β behaviors on phospholipid membranes were observed by Membrane Surface-Enhanced Raman Spectroscopy (MSERS) method. Phospholipid (PL) membranes, consisting of DMPC and DMPS with a molar ratio of 9:1, were fabricated on gold nanoparticles with diameter of 100 nm (Au@PL). Enhancement of the Raman intensity of Au@PL was increased by A β, with enhancement factor about 40. The H-bonding network was disturbed in presence of NaCl which covered Au@PL and made Au@PL away from one another. When A β was applied with Au@PL, the H-bonding network was disturbed just after mixing. As the reaction reaches to equilibrium, A β attracted neighbouring Au@PL and induced aggregation of Au@PL which blocked the aggregation prone site of A β to inhibit further fibrillation. Based on our method, the A β behaviors at lipid membrane surface can be directly observed via enhanced Raman signals.