Ammonium Ion Exchanged Zeolite for Laser Desorption/Ionization Mass Spectrometry of Phosphorylated Peptides. (13th September 2015)

• Record Type: Journal Article
• Authors: Yang, Mengrui; Fujino, Tatsuya
• Other Names: Foret Frantisek Academic Editor.
• Is Part Of: International journal of analytical chemistry. Volume 2015(2015)
• Publication Date: 2015-09-13
• Subjects: Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1155/2015/513761
• Languages: English
• ISSNs: 1687-8760

Abstract

Abstract : α -Cyano-4-hydroxycinnamic acid (CHCA), an organic matrix molecule for matrix-assisted laser desorption/ionization mass spectrometry, was adsorbed toNH 4 + -type zeolite surface, and this new matrix was used for the detection of low-molecular-weight compounds. It was found that this matrix could simplify the mass spectrum in the low-molecular-weight region and prevent interference from fragments and alkali metal ion adducted species. CHCA adsorbed toNH 4 + -type ZSM5 zeolite (CHCA/NH4 ZSM5) was used to measure atropine and aconitine, two toxic alkaloids in plants. In addition, CHCA/NH4 ZSM5 enabled us to detect phosphorylated peptides; peaks of the protonated peptides had higher intensities than the peaks observed using CHCA only.