Detection of Mycobacterium bovis in Organs of Slaughtered Cattle by DNA-Based Polymerase Chain Reaction and Ziehl-Neelsen Techniques in Bauchi State, Nigeria. (3rd February 2015)

• Record Type: Journal Article
• Authors: Sa'idu, A. S.; Okolocha, E. C.; Dzikwi, A. A.; Kwaga, J. K. P.; Gamawa, A. A.; Usman, A.; Maigari, S. A.; Ibrahim, S.
• Other Names: González-Rey Carlos Academic Editor.
• Is Part Of: Journal of veterinary medicine. Volume 2015(2015)
• Publication Date: 2015-02-03
• Subjects: Veterinary medicine -- Periodicals; 636.08905
• DOI: 10.1155/2015/921868
• Languages: English
• ISSNs: 2356-7708

Abstract

Abstract : Bovine tuberculosis is a chronic, infectious, and contagious zoonotic disease of domestic animals, wild animals, and humans. It also poses a public health threat and economic losses. This study was aimed at determining the prevalence of bovine tuberculosis in slaughtered cattle, based on PM meat inspection, Ziehl-Neelsen staining, and PCR techniques in Bauchi State, Nigeria. A Prospective study was conducted on 800 cattle slaughtered in the three Zonal abattoirs of Bauchi State, Nigeria. One hundred and twenty (15%) tissues from different organs had suspected bTB lesions at PM. Out of the samples examined 35 (29.2%) were AFB positive by ZN and 10 (8.3%) were confirmed positive for M. bovis by PCR, with an overall prevalence of 29.16% and 8.33%, respectively. Female had a higher prevalence rate than male cattle at 16.66% and 12.5 % by ZN and 5.00% and 3.33% by PCR, respectively (P > 0.05, χ 2 = 0.218 ). However, there was a statistically significant association (P < 0.05, χ 2 = 7.002 ) between detection of bTB and the age of cattle. ZN revealed that cattle aged 6 years and above had the highest number of positive bTB cases 67.9%, while cattle aged 3–5 years had the lowest 14.81%. PCR technique revealed that the cattle aged 6 and above years also had the highest percentage positive M. bovis cases of 22.84%, whereas cattle aged 3–5 years had the lowest and the overall prevalence rate of 8.33% . The study found a high infection rate of bTB among cattle and majority of the lesions 54.2% were from lungs. The prevalence of bTB was higher in Bauchi metropolitan abattoir which supplies larger population of the state with beef.