Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity. (14th July 2011)

• Record Type: Journal Article
• Authors: Natalia, Dessy; Kohlmann, Christina; Ansorge-Schumacher, Marion B.; Greiner, Lasse
• Other Names: Canovas Manuel Academic Editor.
• Is Part Of: Biotechnology research international. Volume 2011(2011)
• Publication Date: 2011-07-14
• Subjects: Biotechnology -- Periodicals; Biotechnology; Biotechnology; Periodicals; 660.6
• DOI: 10.4061/2011/478925
• Languages: English
• ISSNs: 2090-3138

Abstract

Abstract : Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α -hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α -hydroxy ketone (R)-2, 2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.