Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. (3rd October 2011)

• Record Type: Journal Article
• Authors: Chimata, Murali Krishna; Chetty, Chellu S.; Suresh, Challa
• Other Names: Roukas Triantafyllos Academic Editor.
• Is Part Of: Biotechnology research international. Volume 2011(2011)
• Publication Date: 2011-10-03
• Subjects: Biotechnology -- Periodicals; Biotechnology; Biotechnology; Periodicals; 660.6
• DOI: 10.4061/2011/323891
• Languages: English
• ISSNs: 2090-3138

Abstract

Abstract : The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp. MK 07 and effect of process variables on enzyme production, was studied in a fermentor. It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0. Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source. Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme. Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C. Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5. Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.