Chemical composition of endemic Stachys subnuda Montbret & Aucher ex Benth. essential oil and its anti-inflammatory and antioxidant activities. Issue 4 (4th July 2019)

• Record Type: Journal Article
• Authors: Sen, Ali; Kurkcuoglu, Mine; Bitis, Leyla; Dogan, Ahmet; Baser, Kemal Husnu Can
• Is Part Of: Journal of essential oil research. Volume 31:Issue 4(2019)
• Page Start: 326
• Page End: 334
• Publication Date: 2019-07-04
• Subjects: Stachys subnuda -- essential oil -- anti-inflammatory activity -- antioxidant activity; Essences and essential oils -- Periodicals; Oils, Volatile -- Periodicals; Plant Oils -- Periodicals; 547.71
• DOI: 10.1080/10412905.2019.1567399
• Languages: English
• ISSNs: 1041-2905

Abstract

ABSTRACT: The essential oil of aerial parts of Stachys subnuda Montbret & Aucher ex Benth ( S. subnuda ) was analyzed by gas chromatography (GC) and GC/mass spectrometry (MS). Anti-inflammatory activity was evaluated by lipoxygenase inhibition assay. The antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) assays. Sixty-two constituents, comprising 97.1% of the total essential oil composition, were identified. Phytol (25.2%), β-caryophyllene (11.2%), germacrene D (6.9%), and hexadecanoic acid (6.9%) were the major compounds of aerial part essential oils of S. subnuda . The essential oil showed significant radical scavenging activity with 50% inhibition (IC50 ) values of 0.124 and 0.204 mg/mL for ABTS and DPPH radicals, respectively. The essential oil exhibited moderate anti-inflammatory activity with an IC50 value of 0.419 mg/mL. The results indicated that S. subnuda essential oil had good anti-inflammatory and antioxidant activity. Also, this is first study on chemical composition, anti-inflammatory and antioxidant activity of S. subnuda essential oil.