A direct time-based ITC approach for substrate turnover measurements demonstrated on human FMO3. Issue 44 (10th May 2019)

• Record Type: Journal Article
• Authors: Catucci, Gianluca; Sadeghi, Sheila J.; Gilardi, Gianfranco
• Is Part Of: Chemical communications. Volume 55:Issue 44(2019)
• Page Start: 6217
• Page End: 6220
• Publication Date: 2019-05-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc01356c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Using human FMO3 we demonstrate how time-based ITC can be exploited for measuring kinetic constants and the inhibiton of the conversion of the key substrate trimethylamine into trimethylamine N -oxide. Abstract : Transient binding events are a challenging issue in enzymology. Here we demostrate a time-based ITC approach to human flavin-containing monooxygenase 3, an important drug metabolising enzyme. We measure kinetic constants and we demonstrate how this approach can be exploited for measuring the inhibiton of the conversion of the key substrate trimethylamine into trimethylamine N -oxide.