Effects of acanthoic acid on TNF-α gene expression and haptoglobin synthesis. Issue 4 (1998)

• Record Type: Journal Article
• Authors: Kang Kang, H-S. H-S.; Song Song, H. K. H. K.; Lee Lee, J-J. J-J.; Pyun Pyun, K-H. K-H.; Choi Choi, I. I.
• Is Part Of: Mediators of inflammation. Volume 7:Issue 4(1998)
• Page Start: 257
• Page End: 259
• Publication Date: 1998
• Subjects: Acanthoic acid -- TNF-α -- Haptoglobin; Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/09629359890938
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Tumour necrosis factor-α (TNF-α) is a major proinflammatory cytokine inducing the synthesis and release of many inflammatory mediators. It is involved in immune regulation, autoim mune diseases, and inflammation. Our previous study demonstrated that acanthoic acid, (-)-pimara-9(11), 15-dien19-oic acid, a pimaradiene diterpene isolated from Acanth opanax koreanum, inhibited TNF-α production. To extend our understanding of inhibitory effects of acanthoic acid on TNF-α production, its effects on TNF-α gene expression was tested. Based on the results from RT-PCR and promoter analysis of TNF-α, it was found that acanthoic acid suppressed TNF-α gene expression. But the same concentration of acanthoic acid had no effect on IL-6 gene expression. Haptoglobin is an acute phase protein which is induced by TNF-α. When liver cells were treated with acanthoic acid, haptoglobin synthesis was blocked by acanthoic acid. These data confirmed that acanthoic acid inhibited gene expression and biological function of TNF-α.