Allyl-isothiocyanate ameliorates the pre-neoplastic changes induced by the fern Dryopteris nigropalaceae on experimental feeding in Guinea pigs. (June 2019)

• Record Type: Journal Article
• Authors: Rai, Sarvesh K.; Sharma, Rinku; Patil, Rajendra D.; Periasamy, Sivakumar; Rasmussen, Lars H.; Padwad, Yogendra S.; Kumari, Alka; Bhar, Rasbehari
• Is Part Of: Research in veterinary science. Volume 124(2019)
• Page Start: 24
• Page End: 31
• Publication Date: 2019-06
• Subjects: Enzootic bovine haematuria -- Dryopteris nigropalaceae -- Urinary bladder pre-neoplasia -- Mutations -- Allyl isothiocyanate -- Guinea pig; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2019.02.003
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Enzootic bovine haematuria, caused by long-term ingestion of ferns, is a chronic disease of hill cattle characterized by neoplastic lesions in the urinary bladder. Objectives of this study were to investigate the toxicity potential of long-term feeding of the fern Dryopteris nigropalaceae and effect of allyl isothiocyanate (AITC) to ameliorate fern toxicity and the associated pathological changes. The LC-MS analysis of the fern showed presence of ptaquiloside (4.5 ± 1.0 μg/g) and pterosin B (39 ± 9.1 μg/g). Groups of animals were fed dried fern powder at the dose of 20% w/w in normal feed and treated with and without AITC at graded doses. Long term feeding of fern induced inflammatory and pre-neoplastic lesions in urinary bladder. The important lesions included cystitis, squamous metaplasia and high-grade dysplasia. Urothelium showed positive immunoreactions for nuclear expression of H-ras and p53 . However, no mutation suggestive of neoplastic change was observed on partial mRNA sequences analyses of exon 2 of H-ras and 5 or 7&8 of p53 genes. Strikingly, AITC showed dose-dependent amelioration of pre-neoplastic changes in fern-fed animals. In conclusion, AITC is shown to limit pre-neoplastic changes caused by D. nigropalaceae feeding in guinea pigs. Highlights: Long-term feeding of fern Dryopteris nigropalaceae induced pre-neoplastic lesions in urinary bladder of Guinea pigs. Mutations were not observed in partial mRNA sequences of exon 2 of H-ras and 5 or 7&8 of p53 genes. AITC showed dose-dependent amelioration of pre-neoplastic changes in fern-fed animals.