1, 4-Dioxane exposure induces kidney damage in mice by perturbing specific renal metabolic pathways: An integrated omics insight into the underlying mechanisms. (August 2019)

• Record Type: Journal Article
• Authors: Qiu, Jingfan; Cheng, Jiade; Xie, Yanci; Jiang, Liujing; Shi, Peng; Li, Xinying; Swanda, Robert V.; Zhou, Jun; Wang, Yong
• Is Part Of: Chemosphere. Volume 228(2019)
• Page Start: 149
• Page End: 158
• Publication Date: 2019-08
• Subjects: 1, 4-Dioxane -- Kidney damage -- Arginine metabolism -- Transcriptome -- Metabolome; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.04.111
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: 1, 4-Dioxane (dioxane), an industrial solvent widely detected in environmental and biological matrices, has potential nephrotoxicity. However, the underlying mechanism by which dioxane induces kidney damage remains unclear. In this study, we used an integrated approach, combining kidney transcriptomics and urine metabolomics, to explore the mechanism for the toxic effects of dioxane on the mouse kidney. Transcriptomics profiling showed that exposure to 0.5 mg/L dioxane induced perturbations of multiple signaling pathways in kidneys, such as MAPK and Wnt, although no changes in oxidative stress indicators or anatomical pathology were observed. Exposure to 500 mg/L dioxane significantly disrupted various metabolic pathways, concomitantly with observed renal tissue damage and stimulated oxidant defense system. Urine metabolomic analysis using NMR indicated that exposure to dioxane gradually altered the metabolic profile of urine. Within the full range of altered metabolites, the metabolic pathway containing glycine, serine and threonine was the most significantly altered pathway at the early stage of exposure (3 weeks) in both 0.5 and 500 mg/L dioxane-treated groups. However, with prolonged exposure (9 and 12 weeks), the level of taurine significantly decreased after treatment of 0.5 mg/L dioxane, while exposure to 500 mg/L dioxane significantly increased glutathione levels in urine and decreased arginine metabolism. Furthermore, integrated omics analysis showed that 500 mg/L dioxane exposure induced arginine deficiency by perturbing several genes involved in renal arginine metabolism. Shortage of arginine coupled with increased oxidative stress could lead to renal dysfunction. These findings offer novel insights into the toxicity of dioxane. Graphical abstract: Image 1 Highlights: Sustained dioxane exposure caused nephrotoxicity in mice. 0.5 mg/L dioxane induced no clinical change but perturbed signaling pathways. 500 mg/L dioxane induced kidney injury and stimulated the antioxidant system. 500 mg/L dioxane caused shortage of arginine coupled with increased glutathione. Integrated omics offer potential insights for evaluating renal toxicity.