Α-Lipoic acid prevents senescence, cell cycle arrest, and inflammatory cues in fibroblasts by inhibiting oxidative stress. (March 2019)

• Record Type: Journal Article
• Authors: Baeeri, Maryam; Bahadar, Haji; Rahimifard, Mahban; Navaei-Nigjeh, Mona; Khorasani, Reza; Rezvanfar, Mohammad Amin; Gholami, Mahdi; Abdollahi, Mohammad
• Is Part Of: Pharmacological research. Volume 141(2019)
• Page Start: 214
• Page End: 223
• Publication Date: 2019-03
• Subjects: Ac-DEVD-qNA N-acetyl-Asp-Glu-Val-Asp-P nitroanilide -- Ac-LEHD-qNA N-acetyl-Leu-Glu-His-Asp-P-nitroanilide -- DCFDA 2′, 7′–dichlorofluorescin diacetate -- FRAP ferric reducing antioxidant power -- IL-Iβ interleukin-1β -- MTT 3-4, 5 dimethylthiazol-2-Yl-2, 5-diphenyltetrazolium bromide -- REF rat embryonic fibroblast -- ROS reactive oxygen species -- TBA thiobarbituric acid -- TERT telomerase reverse transcriptase -- TNF-α tumor necrosis factor alpha -- TPTZ tri (2-pyridyl)-S-triazine -- TTM total thiol molecules -- α-LA alpha-lipoic acid; Aging -- Cellular senescence -- α -Lipoic acid -- Oxidative stress -- Rat embryonic fibroblast cells -- Cell cycle -- Toxicity; Pharmacology -- Periodicals; Pharmacology -- Periodicals; Research -- Periodicals; Médicaments -- Recherche -- Périodiques; Pharmacologie -- Périodiques; 615.105
• DOI: 10.1016/j.phrs.2019.01.003
• Languages: English
• ISSNs: 1043-6618

Abstract

Graphical abstract: Abstract: Senescence is a process characterized by an irreversible growth arrest in cells and induced by oxidative stress. In the current study, anti-aging potential of a well-known antioxidant, α-lipoic acid (α-LA), in rat embryonic fibroblast (REF) cells was assessed. In this regard, oxidative stress, inflammation, and apoptosis pathways were investigated on REF cells exposed to H2 O2 as a senescence inducer and α-LA as a protective compound. In cells treated with α-LA and H2 O2, level of β-galactosidase, as an aging marker, and oxidative stress biomarkers, were significantly lower than those exposed to H2 O2 only. Furthermore, flow cytometry assay showed that α-LA caused a significant reduction in the number of apoptotic cells via the caspase-dependent pathway. In addition, it could neutralize the inflammatory effects of H2 O2 and attenuated the concentration of inflammatory cytokines. In comparison to H2 O2 group, a significant increase in G0/G1 arrest was observed during cell cycle analysis in cells exposed to H2 O2 and α-LA. The results of this study show that α-LA has beneficial effects on H2 O2 -induced cellular senescence. α-LA works by attenuating the reactive oxygen species, subsiding inflammation, and affecting cell division.