Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity. (April 2019)

• Record Type: Journal Article
• Authors: Fernandes, Kenner Morais; Tomé, Hudson Vaner Ventura; Miranda, Franciane Rosa; Gonçalves, Wagner Gonzaga; Pascini, Tales Vicari; Serrão, José Eduardo; Martins, Gustavo Ferreira
• Is Part Of: Chemosphere. Volume 221(2019)
• Page Start: 464
• Page End: 470
• Publication Date: 2019-04
• Subjects: Apoptosis -- Bioinsecticide -- Metamorphosis -- Mosquitoes -- Insecticide toxicity; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.01.068
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here, we investigate the sublethal effects of spinosad during midgut metamorphosis of A. aegypti females and its cumulative effects on blood acquisition capacity and fecundity in adults. We studied the midgut because it is an important model organ directly related to blood acquisition and digestion. Treatment of larvae with spinosad induced oxidative stress, apoptosis, and damage to the midgut cells at all stages of development and in adults. There was a reduction in the number of proliferating cells and the number of enteroendocrine cells in treated individuals. In addition, damage caused by spinosad led to a reduction in oviposition and egg viability of A. aegypti females. Finally, the exposure of mosquito larvae to sublethal concentrations of spinosad interfered with the development of the midgut, arresting the blood digestion and reproduction of adult females with blood digestion and reproduction difficulties. Graphical abstract: Image 1 Highlights: Treatment with spinosad originates Aedes aegypti females with low fecundity. Treatment with spinosad interferes with the development of the midgut of mosquito. Treatment with spinosad induces oxidative stress and apoptosis in the midgut.