Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep. (June 2019)

• Record Type: Journal Article
• Authors: Cancellara, Lina; Quartesan, Silvia; Toniolo, Luana; Reggiani, Carlo; Mascarello, Francesco; Melotti, Luca; Francolini, Maura; Maccatrozzo, Lisa; Patruno, Marco
• Is Part Of: Research in veterinary science. Volume 124(2019)
• Page Start: 270
• Page End: 279
• Publication Date: 2019-06
• Subjects: Muscle involution -- Myosin heavy chain isoforms -- Proximal sesamoidean ligament -- Pax7 -- Wnt signalling -- Myogenic regulatory factors; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2019.04.006
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180 days and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. We observed an accelerated maturation, as indicated by the transition of MyHC isoform expression towards the slow isoforms and a reduced regenerative potential indicated by the low Pax7 expression and the altered Wnt signalling. We also found a specific myogenic expression pattern of MyoD, Myf5 and Myogenin in the developing PSL and high mRNA levels of specific fibrogenic factors, as TGF-β1, that, undoubtedly, stimulate the growth of connective tissue. Our observations confirmed, at molecular level, the peculiarity of the fast involution observed in PSL a muscle that undergoes a very specific active differentiation process during early development, which implies myofibres involution and their replacement with connective tissue. Highlights: We studied the development of the Proximal Sesamoidean Ligament (PSL) that undergoes a premature involution. We found a specific myogenic pattern that drives the accelerated involution of PSL. TGF-β1 stimulate the growth of connective tissue. PSL could be considered an interesting model for understanding involution mechanisms.