MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation. (10th April 2019)

• Record Type: Journal Article
• Authors: Sul, Ok-Joo; Rajasekaran, Monisha; Park, Hyun-Jung; Suh, Jae-Hee; Choi, Hye-Seon
• Other Names: Morishita Ryuichi Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2019(2019)
• Publication Date: 2019-04-10
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2019/6018180
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : Recent findings suggest that microRNAs (miRs) play a critical role in osteoclastogenesis, which regulates bone loss. We hypothesized that inflammation induces miR-29b, which increases the survival rate in osteoclasts (OCs), leading to bone loss. The expression level of miR-29b increased in OC stimulated by lipopolysaccharide (LPS) in an in vitro system which correlated with its increase in tibiae from mice that received LPS injections compared with those that received vehicle treatment. An miR-29b mimic increased OC survival rate without any change in OC differentiation, and furthermore, the inhibition of endogenous miR-29b induced by LPS decreased OC survival rate. Increased OC survival rate after overexpression of miR-29b was associated with antiapoptotic activity, as shown by staining annexin V-positive cells. We found that a target gene of miR-29b is BCL-2-modifying factor ( Bmf ), which acts as a proapoptotic factor, and that miR-29b binds to the 3 ′ -UTR of Bmf . Our data demonstrate that LPS-induced miR-29b increases the number of OC by enhancing OC survival through decreased BMF.