Effect of Novel Marine Nutraceuticals on IL-1α-Mediated TNF-α Release from UVB-Irradiated Human Melanocyte-Derived Cells. (1st August 2011)

• Record Type: Journal Article
• Authors: Muthusamy, Visalini; Hodges, Lynn D.; Macrides, Theodore A.; Boyle, Glen M.; Piva, Terrence J.
• Other Names: Signorini Cinzia Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2011(2011)
• Publication Date: 2011-08-01
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2011/728645
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5 β -scymnol and CO2 -supercritical fluid extract (CO2 -SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor- α (TNF- α ), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1 α . Cell viability and TNF- α levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15 min after-irradiation. When α -tocopherol, CO2 -SFE mussel oil, and 5 β -scymnol were added to the UVB-irradiated HEM cells treated with IL-1 α, TNF- α levels fell by 53%, 65%, and 76%, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes.