Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis. (22nd February 2016)

• Record Type: Journal Article
• Authors: Khan, Hamayun; Amin, Hazrat; Ullah, Asad; Saba, Sumbal; Rafique, Jamal; Khan, Khalid; Ahmad, Nasir; Badshah, Syed Lal
• Other Names: Delic Denis Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2016(2016)
• Publication Date: 2016-02-22
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2016/1051925
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : Two important biologically active compounds were isolated from Mallotus philippensis . The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1 ) and 11 -O -galloylbergenin (2 ). The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA), reducing power assay (RPA), and total antioxidant capacity (TAC) assay, were adopted. The results showed that compound2 was found to be more potent antioxidant as compared to1 . Fascinatingly, compound2 displayed better EC50 results as compared toα -tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain of Plasmodium falciparum (D10) and IC50 values were found to be less than 8 μ M. The in silico molecular docking analyses were also performed for the determination of binding affinity of the isolated compounds using P. falciparum proteins PfLDH and Pfg27. The results showed that compound2 has high docking score and binding affinity to both protein receptors as compared to compound1 . The demonstrated biological potentials declared that compound2 could be the better natural antioxidant and antiplasmodial candidate.