Construction of a novel xanthine biosensor using zinc oxide (ZnO) and the biotemplate method for detection of fish freshness. Issue 8 (1st February 2019)

• Record Type: Journal Article
• Authors: Xue, Gao; Yu, Wang; Yutong, Liu; Qiang, Zhang; Xiuying, Liu; Yiwei, Tang; Jianrong, Li
• Is Part Of: Analytical methods. Volume 11:Issue 8(2019)
• Page Start: 1021
• Page End: 1026
• Publication Date: 2019-02-01
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay02554a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : In the food industry, fish is a product that has a short shelf life, and xanthine has attracted significant attention as an indicator of the freshness of fish. Abstract : In the food industry, fish is a product that has a short shelf life, and xanthine has attracted significant attention as an indicator of the freshness of fish. In this study, we demonstrated a simple assay system containing zinc oxide (ZnO) and xanthine oxidase (XOD) for specific and quantitative detection of xanthine. ZnO nanomaterials have been successfully synthesized using a biotemplate method. The mechanism is based on the fluorescence quenching of ZnO via the electron transfer mechanism, which is caused by the hydrogen peroxide (H2 O2 ) that is produced from the XOD-catalyzed oxidation of xanthine. By virtue of the specific response, the present assay allowed for the selective determination of xanthine in the range of 3.30 × 10 −10 –6.67 × 10 −7 mol L −1 and 2.67 × 10 −6 –2.67 × 10 −4 mol L −1 with a detection limit of 1.30 × 10 −10 mol L −1 . The proposed method was successfully applied to the determination of xanthine in real fish samples with satisfactory results.