Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture. Issue 5 (21st January 2019)

• Record Type: Journal Article
• Authors: Xu, Xiaomin; Chen, Jiyun; Li, Bangrui; Tang, Lijuan; Jiang, Jianhui
• Is Part Of: Analyst. Volume 144:Issue 5(2019)
• Page Start: 1725
• Page End: 1730
• Publication Date: 2019-01-21
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an02063a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. Abstract : Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.